Research & Initiatives
Our Research Focus
We're particularly interested in special proteins called transcription factors that control gene activity. Think of these as the cell's master controllers – they decide which genes get turned on or off. Many of these controllers have flexible, "shape-shifting" regions that allow them to form droplets-like structures which can control cellular signaling processes. When mutations occur in these regions, it can lead to various diseases, including different types of cancer.
These control centers are fascinating because they work like cellular command posts, gathering multiple proteins and molecules together to coordinate gene regulation. Just as a conductor coordinates an orchestra to create harmonious music, these droplet-forming transcription factors orchestrate complex patterns of gene activity. When mutations disrupt this process, it's like having a conductor giving wrong signals to the orchestra – the resulting chaos can drive disease development.
Our research investigates how these molecular conductors normally work, what happens when they malfunction, and how we might develop new therapies by targeting these droplet-forming processes. By understanding these fundamental mechanisms, we aim to find new ways to treat diseases where gene regulation goes awry.
Advanced microscopy to visualize droplet formation
Advanced microscopy techniques, such as confocal, super-resolution, or cryo-electron microscopy, enable real-time visualization of droplet formation at high spatial and temporal resolution. These methods reveal the dynamics of phase separation, droplet size, morphology, and interactions with surrounding environments. By capturing detailed images, researchers can study how droplets nucleate, grow, and evolve under different conditions. This visual data is crucial for understanding the physical principles governing droplet behavior.
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Protein engineering to understand droplet properties
Protein engineering involves designing and modifying proteins to explore their role in droplet formation and properties. By altering amino acid sequences or domains, researchers can investigate how specific protein features influence phase separation, stability, and material properties of droplets. This approach helps identify key molecular drivers of droplet formation and provides insights into how mutations or environmental changes affect droplet behavior. Engineered proteins also serve as tools to probe the functional implications of droplets in cellular processes.
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Mass spectrometry to identify droplet components
Mass spectrometry is a powerful analytical tool used to identify and quantify the molecular components within droplets. By analyzing the composition of droplets, researchers can determine which proteins, nucleic acids, or other biomolecules are enriched or excluded. This information helps uncover the molecular mechanisms underlying droplet formation and function. Additionally, mass spectrometry can reveal post-translational modifications or interactions that influence droplet properties.
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Computational analysis to predict droplet behavior
Computational analysis involves using simulations, machine learning, and theoretical models to predict droplet behavior and phase separation. These tools can simulate molecular interactions, droplet dynamics, and environmental effects, providing insights that are difficult to obtain experimentally. Computational approaches also help identify key parameters governing droplet formation and stability. By integrating experimental data, these models enhance our ability to design and control droplets for various applications.
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